RADIATION-INDUCED KI-67 PROLIFERATION IN THE TESTES OF LABORATORY RATS

The dominant role of the neutron-activated radionuclide - Manganese-56 (⁵⁶Mn) was noted in the scientific papers studying the effects of atomic bombing in Japanese cities, which deserves interest nowadays. Among the available literature, there are insufficient works devoted to the study of general patterns and possible mechanisms of proliferative activity of Ki-67 in testicular tissue under inhalation exposure to neutron rays. Animals were subjected to necropsy on the 3^rd, 14^th and 60^th days after irradiation, then the testes were removed, after which it was fixed in 10% formalin. Paraffin sections were dewaxed and rehydrated according to standard procedures. Immunohistochemical reaction was visualized using the DAB + (DAKO) system. The number of proliferative activity marker, namely Ki-67 was calculated taking into account the stained nuclei of any degree of intensity, expressing the results in percent. All quantitative variables studied were described using mean (m), median (Me), and interquartile range (IQR). When comparing them, depending on the studied factors, the Kruskal-Wallis test (SPSS 2.0) was used. In a comparative aspect of⁵⁶Mn, Mn0₂ and⁶⁰Co effect on the organism, a high level of Ki-67 antigen in the testicular tissues studied was observed in experimental animals at a later date after neutron-activated manganese dioxide exposure.

⁵⁶Mn, testes, immunohistochemistry, Ki-67, rats

1. Анискина А. И. Влияние аминокислот на клеточную пролиферацию и апоптоз в органической культуре тканей молодых и старых крыс /А. И. Анискина, Н. И. Чалисова, А. Н. Закуцкий //Успехи геронтологии. - 2006. -№19. - С. 55-59.

2. Рахыпбеков Т. K. Радиационно-биологический эксперимент на комплексе исследовательских реакторов «Байкал-1» /Т. K. Рахыпбеков, М. Хоши, В. Ф. Степаненко //Человек. Энергия. Атом. - 2015. - №2(24). - С. 43-45.

3. Agarwal A. The role of free radicals and antioxidants in reproduction /A. Agarwal, S. Gupta, S. Sikka //Current Opinion in Obstetrics and Gynecology. - 2006. - V. 18, №3. - P. 325-332.

4. Albuquerque A. V. Spermatogonial behavior in rats during radiation-induced arrest and recovery after hormone suppression /A. V. Albuquerque, F. R. Almeida, C. C. Weng //Reproduction. - 2013. - V. 146, №4. - P. 63-76.

5. Dabbs D. J. Diagnostic Immunihisto-chemistry: theranostic and genomic applications. Fourth Edition. - Elsevier Science, 2014. - 931 p.

6. Dhall D. Ki-67 proliferative index predicts progression-free survival of patients with well-differentiated ileal neuroendocrine tumors / D. Dhall, R. Mertens, C. Bresee //Human Pathology. - 2012. - V. 43, №4. - P. 489495.

7. Gallegos I. Immunohistochemistry expression of P53, Ki67, CD30, and CD117 and presence of clinical metastasis at diagnosis of testicular seminoma /I. Gallegos, J. P. Valdevenito, R. Miranda //Applied Immuno histochemistry and Molecular Morphology. - 2011. -V. 19, №2. - P. 147152.

8. Gu C. H. Over-expression of testis-specific expressed gene 1 attenuates the proliferation and induces apoptosis of GC-1spg cells /C. H. Gu, F. Y. Tian, J. R. Pu //Journal of Huazhong University of Science and Technology. Medical Sciences. - 2014. - V. 34, №4. - P. 535-541.

9. Lee W. Protective Effect of administered rolipram against radiation-induced testicular injury in mice /W. Lee, Y. Son, H. Jang //The World Journal of Men's Health. - 2015. - V. 33, №1. - P. 20-29.

10. Luo Q. The effect of Lycium barbarum polysaccharides on the male rats 'reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation /q. Luo, J. Li, X. Cui //Journal of Ethnopharmacology. - 2014. - V. 154, №1. - P. 249-258.

11. Pei R. Prognostic value of Ki-67 expression in patients with extensive-stage small cell lung cancer /R. Pei, L. Zhang, C. Xie //Future Oncology. - 2017 - V. 13, №14. - P. 1247-1252.

12. Schmitz-Feuerhake I. Genetic radiation risks: a neglected topic in the low dose debate /I. Schmitz-Feuerhake, C. Busby, S. Pflugbeil // Environmental Health and Toxicology. - 2016. -V. 31. - 2001 p.

13. Uzbekov D. Rаdiаtiоn еffect оn mоrphоlogy stаtе оn the mаlе rеprоductivе system /D. Uzbekov, M. Hoshi, N. Chaizhunusova //Astana medical journal. - 2017. - №1(91). - P. 94-100.

14. Viale G. Predictive value of tumor Ki-67 expression in two randomized trials of adjuvant chemoendocrine therapy for nodenegative breast cancer /G. Viale, M. M. Regan, M. G. Mastropasqua //Journ. of the National Cancer Institute. - 2008. - V. 100, №3. - Р. 207-212.

15. Yerushalmi R. Ki-67 in breast cancer: prognostic and predictive potential /R. Yerushalmi, R. Woods, P. M. Ravdin //The Lancet Oncology. - 2010. - V. 11, №2. - Р. 174-183.