medecolМедицина и экологияMedicine and ecology2305-60452305-6053Карагандинский медицинский университет10.59598/ME-2305-6053-2025-114-1-126-136medecol-928Research ArticleТЕОРЕТИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАTHEORETICAL AND EXPERIMENTAL MEDICINEОценка эффективности остеорегенерации при использовании rhBMP-2 и костного аллографта на модели дефекта бедренной кости у кроликовEvaluation of the effectiveness of osteoregeneration using rhBMP-2 and bone allograft on a model of femoral defect in rabbitsТулеубаевБ. Е.TuleubaevB. E.

100008, г. Караганда, ул. Гоголя 40

100008, Karaganda, Gogolya str., 40

info@qmu.kzДарыбаевД. М.DarybaevD. M.

Дарын Маратұлы Дарыбаев – докторант 3 курса специальности 8D10100 – Медицина

100008, г. Караганда, ул. Гоголя 40

Daryn Maratuly Darybaev – the 3rd year doctoral student of the Medicine 8D10100 specialty

100008, Karaganda, Gogolya str., 40

Darybaev@qmu.kzКошановаА. А.KoshanovaA. A.

100008, г. Караганда, ул. Гоголя 40

100008, Karaganda, Gogolya str., 40

info@qmu.kzАвромидиИ. К.AvromidiI. K.

100008, г. Караганда, ул. Гоголя 40

100008, Karaganda, Gogolya str., 40

info@qmu.kzНАО «Медицинский университет Караганды»КазахстанKaraganda Medical University NC JSCKazakhstan20251605202501126136Copyright © Тулеубаев Б.Е., Дарыбаев Д.М., Кошанова А.А., Авромиди И.К., 20252025Тулеубаев Б.Е., Дарыбаев Д.М., Кошанова А.А., Авромиди И.К.Tuleubaev B.E., Darybaev D.M., Koshanova A.A., Avromidi I.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medecol.qmu.kz/jour/article/view/928Цель

Цель. Гистологическая и гистоморфометрическая оценка процессов регенерации костной ткани, при использовании костного аллографта заготовленного по Марбургской системе костного банка в сочетании с rhBMP-2 на модели бедренного дефекта у кролика.

Материалы и методы

Материалы и методы. В 2 группах экспериментальных животных, по 24 испытуемых (кроликов)в каждой, в дистальном метафизе бедренной кости формировали дефект диаметром 5 мм. В группе 1 (AG+rhBMP-2) дефект заполняли костным аллографтом в сочетании с rhBMP-2, во группе 2 (AG) дефект заполняли костным аллографтом без дополнительных препаратов. Далее проводилась гистологическая и статистическая обработка полученных данных.

Результаты

Результаты. На 14 сут в группе AG+rhBMP-2 новообразованная костная ткань составила 34.75±5.67% от общей площади дефекта кортикальной пластинки. На 30 сут в группе AG+rhBMP-2 закрытие дефекта кортикальной пластинки составило 79.12±14.32%, новообразованная костная ткань закрывала 80.75% длины и 71.37% толщины кортикальной пластинки. На 60 сут в группе AG+rhBMP-2 новообразованная костная ткань закрывала общую площадь дефекта кортикальной пластинки на 64.50±27.73%, длину – на 62%, толщину – на 65.25%.

Обсуждение

Обсуждение. Результаты свидетельствуют об ускорении остеорегенерации на ранних этапах восстановления костной пластинки в группе AG+rhBMP-2, однако результаты, полученные к 60 сут, были крайне неоднородными, что указывало на диссенергичное влияние rhBMP-2 на мезенхимальные клетки-предшественники и связанный с этим избыточный резорбтивный эффект rhBMP-2 в некоторых случаях.

Заключение

Заключение. Применение костного аллографта в сочетании с rhBMP-2 на поздних этапах регенерации костной ткани приводит к неудовлетворительным результатам, что проявляется в виде высокой неоднородности получаемых данных, среди которыхв значительной части случаев не происходит достаточного закрытия дефекта новообразованной костной тканью.

Aim

Aim. To carry out a histological and histomorphometric assessment of bone tissue regeneration processes using a bone allograph prepared according to the Marburg bone bank system in combination with rhBMP-2 on a rabbit femoral defect model.

Materials and methods

Materials and methods. In this study, 2 groups were formed, each with 24 subjects (rabbits), who had a defect with a diameter of 5 mm in the distal metaphysis of the femur. In group 1, the defect was filled with bone allograft in combination with rhBMP-2. In group 2, the defect was filled with bone allograft without additional drugs. Further, histological and statistical processing of the obtained data was carried out.

Results

Results. On day 14, in the AG+rhBMP-2 group, newly formed bone tissue amounted to 34.75±5.67% of the total area of the cortical plate defect. On day 30, in the AG+rhBMP-2 group, the closure of the cortical plate defect was 79.12±14.32%, the newly formed bone tissue covered 80.75% of the length and 71.37% of the thickness of the cortical plate. On day 60 in the AG+rhBMP-2 group, newly formed bone tissue covered the total area of the cortical plate defect by 64.50±27.73%, length by 62%, and thickness by 65.25%.

Discussion

Discussion. The data obtained indicate an acceleration of osteoregeneration in the early stages of bone plate restoration in the AG+rhBMP-2 group, however, the results obtained by 60 days were extremely heterogeneous, indicating the dysenergic effect of rhBMP-2 on mesenchymal progenitor cells and the associated excessive resorptive effect of rhBMP-2 in some cases.

Conclusion

Conclusion. According to the results of this study, it can be seen that the use of bone allograft in combination with rhBMP-2 at the late stages of bone regeneration leads to unsatisfactory results, which manifests itself in the form of high heterogeneity of the data obtained, among which, in a significant part of cases, there is not sufficient closure of the defect with newly formed bone tissue.

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The authors declare that there are no conflicts of interest present.