medecolМедицина и экологияMedicine and ecology2305-60452305-6053Карагандинский медицинский университет10.59598/ME-2305-6045-2023-108-3-34-38medecol-425Research ArticleТЕОРЕТИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАTHEORETICAL AND EXPERIMENTAL MEDICINEСинтез и характеристика карбоксиметилцеллюлозы из сосновой хвои для применения в биомедицине и регенеративной медицинеSynthesis and characterization of carboxymethyl cellulose from pine needles for biomedical and regenerative medicine applicationsБхадриН.BhadriN.

Уттаракханд

Uttarakhand

БхаттП.BhattP.

Уттаракханд

Uttarakhand

БартвалА.BarthwalA.

Уттаракханд

Uttarakhand

ПхулараС. Ч.PhularaS. Ch.

Суреш Чандра Пхулара – доцент кафедры биотехнологии 

Уттаракханд

Dr. Suresh Chandra Phulara – Assistant Professor

Uttarakhand

phulara.biotech@gmail.comФакультет биотехнологии Инженерно-технологического института Говинда Баллабха Панта, Паури Гархвал-246194ИндияDepartment of Biotechnology, Govind Ballabh Pant Institute of Engineering and Technology, Pauri Garhwal-246194India202319102023033438Copyright © Бхадри Н., Бхатт П., Бартвал А., Пхулара С.Ч., 20232023Бхадри Н., Бхатт П., Бартвал А., Пхулара С.Ч.Bhadri N., Bhatt P., Barthwal A., Phulara S.C.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medecol.qmu.kz/jour/article/view/425

Карбоксиметилцеллюлоза (КМЦ) является одним из наиболее широко используемых компонентов гидрогеля для биомедицинских применений, поскольку ее предшественница целлюлоза является самой распространенной биологической макромолекулой на Земле. В последние годы наблюдается заметный рост синтеза КМЦ из различных растительных источников. В настоящем исследовании использовали хвою бодрящей сосны (Pinus spp.) из-за ее обилия и легкой доступности в гималайских регионах по всей Индии. Целлюлоза была извлечена из сосновых иголок. Затем процесс этерификации был использован для получения КМЦ из целлюлозы сосновых иголок с гидроксидом натрия и монохлоруксусной кислотой. Выход КМЦ, степень замещения, влагоудерживающая способность, маслоудерживающая способность, зольность и содержание КМЦ определяли путем сравнения полученной КМЦ со стандартной КМЦ. Чистота синтезированной КМЦ определяется содержанием КМЦ. Полученная КМЦ имела чистоту 10 % и выход 110 %. Степень замещения, влагоудерживающая способность и маслоудерживающая способность были определены как равные 0,02, 8,4 г/г и 4 г/г соответственно. Содержание золы в синтезированной КМЦ, по расчетам, составляло 6 %. Большинство характеристик были либо эквивалентны коммерческой КМЦ, либо превосходили ее, поэтому синтезированный продукт может быть использован в качестве потенциального компонента для изготовления биоматериалов для биомедицинских применений.

Carboxymethyl cellulose (CMC) is one of the most widely used components of hydrogel for biomedical applications as its precursor cellulose is the most abundant biological macromolecule on the Earth. Recent years have seen a remarkable increase in CMC synthesis from various plant sources. In the present study we have utilised cheer pine needles (Pinus spp.) due to its abundance and easy availability in Himalayan regions across India. The cellulose was extracted from pine needles. Then, the etherification process was used to prepare CMC from pine needle cellulose, with sodium hydroxide and monochloroacetic acid (MCA). CMC yield, degree of substitution, water retention capacity, oil retention capacity, ash content, and CMC content were determined by comparing obtained CMC to standard CMC. The purity of the synthesised CMC is represented by CMC content. The prepared CMC had a purity of 10 % and a yield of 110 %. The degree of substitution, water retention capacity, and oil retention capacity were all determined to be 0.02, 8.4 g/g, and 4g/g, respectively. The ash content of the synthesised CMC was calculated to be 6 %. The majority of characteristics were either equivalent to commercial CMC or superior to it therefore the synthesised product can be used as a potential component to fabricate biomaterials for biomedical applications.

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The authors declare that there are no conflicts of interest present.