medecolМедицина и экологияMedicine and ecology2305-60452305-6053Карагандинский медицинский университетmedecol-256Research ArticleТЕОРЕТИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАTHEORETICAL AND EXPERIMENTAL MEDICINEОСНОВЫ КЛЕТОЧНЫХ ТЕХНОЛОГИЙFUNDAMENTALS OF CELL CULTUREСэндлТ.SandleT. .noemail@neicon.ruЧескаА.ChescaA. .noemail@neicon.ruАбдулинаГ.AbdulinaG. .noemail@neicon.ruУниверситет МанчестераСоединённые Штаты АмерикиManchester UniversityUnited StatesУниверситет Трансильвании г. БрашовРумынияTransilvania University of BrasovRomaniaКарагандинский медицинский университетКазахстанKaraganda Medical UniversityKazakhstan202111102021016069Copyright © Сэндл Т..., Ческа А..., Абдулина Г..., 20212021Сэндл Т., Ческа А., Абдулина Г.Sandle T..., Chesca A..., Abdulina G...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medecol.qmu.kz/jour/article/view/256

Цель настоящей работы - дать студентам, врачам, магистрам фундаментальное представление о клеточных технологиях. Культура клеток - важный метод как в клеточной, так и в молекулярной биологии, поскольку он обеспечивает лучшую платформу для изучения нормальной физиологии и биохимии клеток. Причина сосредоточения внимания на системах клеточных культур заключается в том, что они являются незаменимыми инструментами для фундаментальных исследований и широкого спектра клинических исследований in vitro. В статье дается критический анализ: как положительные, так и отрицательные аспекты клеточных технологий. Клеточные культуры могут быть использованы для имитации условий в живом организме. К недостаткам можно отнести то, что культура клеток все же не абсолютная модель живого организма, а лишь напоминает условия жизни живых организмов. Еще один недостаток связан с загрязнением клеточной культуры; можно выделить химические и биологические загрязнители. Работа с культурой клеток - это финансово затратный трудоемкий процесс: требует наличия современного оборудования, профильного помещения, хорошо обученного персонала. В статье подробно рассматриваются вопросы применения клеточных культур в медицине, фармацевтическом производстве и биологии

The purpose of this review is to provide a fundamental understanding of cellular technologies for students, doctors, masters. Cell culture is an important technique in both cellular and molecular biology given that it provides the best platform for studying the normal physiology and biochemistry of cells. The reason for focusing on cell culture systems is because they are indispensable tools for basic research and a wide range of clinical in vitro studies. The review provides a critical analysis: both positive and negative aspects of cell technologies. Cell cultures can be used to mimic living conditions. The disadvantages include the fact that cell culture is still not an absolute model of a living organism, but only resembles the living conditions of living organisms. A further disadvantage is with contaminants of cell culture; these can be divided into chemical and biological contaminants. Working with cell culture is a financially costly laborious process: it requires modern equipment, specialized laboratory and well trained staff. The article discusses in detail the issues of the use of cell cultures in medicine, pharmaceutical industry and biology.

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The authors declare that there are no conflicts of interest present.