medecolМедицина и экологияMedicine and ecology2305-60452305-6053Карагандинский медицинский университет10.59598/ME-2305-6053-2026-120-3-116-124medecol-1381Research ArticleТЕОРЕТИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАTHEORETICAL AND EXPERIMENTAL MEDICINEЗащитное действие масла крапивы на окислительный стресс и воспаление в головном мозге крыс после воздействия гамма-излучения и шестивалентного хромаProtective effects of Urtica dioica L. oil on oxidative stress and inflammation in the rat brain following gamma irradiation and hexavalent chromium exposureАбугалиеваН. С.AbugaliyevaN. S.

Назерке Сериковна Абугалиева

030017, г Актобе, ул. Жанаконыс, 8Е

Nazerke Serikkyzy Abugaliyeva 

030017, Aktobe c., Zhanakonys str., 8Е

aktobeob@mail.ruИзтлеуовМ. К.IztleuovM. K.

030000, г Актобе, ул. Маресьева, 68

030000, Aktobe с., Mareseva str., 68

info@zkmu.kzИзтлеуовЕ. М.IztleuovY. M.

030000, г Актобе, ул. Маресьева, 68

030000, Aktobe с., Mareseva str., 68

info@zkmu.kzРыжкинС. А.RyzhkinS. A.

125993, Москва, ул. Баррикадная, 2/1

125993, Moscow c., Barrikadnaya str., 2/1

rsa777@inbox.ruСмагуловаГ. А.SmagulovaG. A.

030000, г Актобе, ул. Маресьева, 68

030000, Aktobe с., Mareseva str., 68

info@zkmu.kzСапарбаевС. С.SaparbaevS. S.

020000, г. Астана, ул. Бейбит Майлин, 31

020000, Astana c., Beibit Mailin str., 31

al-zhami@mail.ruАктюбинская областная многопрофильная больницаКазахстанAktobe Regional Multidisciplinary HospitalKazakhstanКафедра естественных наук, НАО «Западно-Казахстанский медицинский университет им. Марата Оспанова»КазахстанDepartment of Natural sciences, NC JSC «Marat Ospanov West Kazakhstan Medical University»KazakhstanКафедра лучевой диагностики, НАО «Западно-Казахстанский медицинский университет им. Марата Оспанова»КазахстанDepartment of Radiation diagnostics, NC JSC «Marat Ospanov West Kazakhstan Medical University»KazakhstanКафедра радиологии, радиотерапии, радиационной гигиены и радиационной безопасности, Российская медицинская академия непрерывного профессионального образованияРоссияDepartment of Radiology, Radiotherapy, Radiation Hygiene and Radiation Safety, Russian Medical Academy of Continuous Professional EducationRussian FederationКафедра фармакологии, НАО «Западно-Казахстанский медицинский университет им. Марата Оспанова»КазахстанDepartment of Pharmacology, NC JSC «Marat Ospanov West Kazakhstan Medical University»KazakhstanМедицинский центр Аль-ЖамиКазахстанMedical Center Al-JhamiKazakhstan20261210202603116124Copyright © Абугалиева Н.С., Изтлеуов М.К., Изтлеуов Е.М., Рыжкин С.А., Смагулова Г.А., Сапарбаев С.С., 20262026Абугалиева Н.С., Изтлеуов М.К., Изтлеуов Е.М., Рыжкин С.А., Смагулова Г.А., Сапарбаев С.С.Abugaliyeva N.S., Iztleuov M.K., Iztleuov Y.M., Ryzhkin S.A., Smagulova G.A., Saparbaev S.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medecol.qmu.kz/jour/article/view/1381Цель

Цель. Оценить нейропротекторное действие масла крапивы (Urtica dioica L.) на окислительный стресс и воспалительные реакции в головном мозге крыс, подвергшихся воздействию ионизирующего излучения отдельно или в сочетании с шестивалентным хромом.

Материалы и методы

Материалы и методы. Шестьдесят пять белых взрослых самцов крыс были случайным образом распределены на пять групп (n=13/группа): контрольная, облучение, облучение + масло крапивы, облучение + Cr(VI) и облучение + Cr(VI) + масло крапивы. Через семь дней после облучения была собрана ткань головного мозга для биохимического анализа. Были определены уровни малонового диальдегида (МДА), восстановленного глутатиона (GSH), супероксиддисмутазы (СОД), каталазы (КАТ), фактора некроза опухолей-α (ФНО-α) и интерлейкина-6 (ИЛ-6).

Результаты и обсуждение

Результаты и обсуждение. Воздействие ионизирующего излучения, особенно в сочетании с Cr(VI), значительно ослабило антиоксидантную защиту, усилило перекисное окисление липидов и обусловило повышение уровня воспалительных цитокинов в ткани головного мозга. Предварительная обработка маслом крапивы значительно стимулировала повышение уровней СОД, КАТ и GSH с одновременным снижением уровней МДА, ФНО-α и ИЛ-6 по сравнению с соответствующими группами, не подвергавшимися воздействию (p<0,05).

Выводы

Выводы. Масло крапивы ослабило окислительный стресс и нейровоспаление, вызванные ионизирующим излучением как отдельно, так и в сочетании с шестивалентным хромом. Эти результаты свидетельствуют о том, что масло крапивы обладает многообещающим нейропротекторным потенциалом за счет усиления эндогенной антиоксидантной защиты и подавления воспалительных реакций.

Aim

Aim. To evaluate the neuroprotective effects of Urtica dioica L. oil on oxidative stress and inflammatory responses in the brains of rats exposed to ionizing radiation alone or in combination with hexavalent chromium.

Materials and methods

Materials and methods. Sixty-five white adult male rats were randomly assigned to five groups (n=13/ group): control, irradiation, irradiation + Urtica dioica L. oil, irradiation + Cr(VI), and irradiation + Cr(VI) + Urtica dioica L. oil. Seven days after irradiation, brain tissue was collected for biochemical analysis. Levels of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were determined.

Results and discussion

Results and discussion. Exposure to ionizing radiation, particularly in combination with Cr(VI), significantly impaired antioxidant defenses and increased lipid peroxidation and inflammatory cytokine levels in brain tissue. Pretreatment with Urtica dioica L. oil significantly increased SOD, CAT, and GSH levels while reducing MDA, TNF-α, and IL-6 compared with the corresponding untreated exposed groups (p<0.05).

Conclusions

Conclusions. Urtica dioica L. oil attenuated oxidative stress and neuroinflammation induced by ionizing radiation alone or combined with hexavalent chromium. These findings suggest that nettle oil has promising neuroprotective potential through enhancement of endogenous antioxidant defenses and suppression of inflammatory responses.

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The authors declare that there are no conflicts of interest present.