medecolМедицина и экологияMedicine and ecology2305-60452305-6053Карагандинский медицинский университет10.59598/ME-2305-6053-2025-115-2-137-150medecol-1005Research ArticleТЕОРЕТИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАTHEORETICAL AND EXPERIMENTAL MEDICINEПредоперационное применение плазмы, обогащенной тромбоцитами, в комбинации с сосудисто-стромальной фракцией в лечении длительно незаживающих ранPREOPERATIVE USE OF PLATELET-RICH PLASMA IN COMBINATION WITH STROMAL VASCULAR FRACTION IN TREATMENT OF LONG NON-HEALING WOUNDSАскаровМ. С.AskarovM. S.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

ПакИ. Л.PakI. L.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

БатеноваУ. Г.BatenovaU. G.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

ЕскермесовД. Б.YeskermessovD. B.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

КлюевД. А.KlyuyevD. A.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

КамышанскийЕ. К.KamyshanskiyYe. K.

100008, г. Караганда, ул. Гоголя, 40

100008, Karaganda, Gogolya str., 40

Кафедра хирургических болезней НАО «Карагандинский медицинский университет»КазахстанDepartment of Surgical Diseases of Karaganda Medical University NC JSCKazakhstanИнститут наук о жизни НАО «Карагандинский медицинский университет»КазахстанInstitute of Life Sciences of Karaganda Medical University NC JSCKazakhstanПатологоанатомический блок клиники НАО «Карагандинский медицинский университет»КазахстанPathological Anatomy Unit of the Clinic of Karaganda Medical University NC JSCKazakhstan20250908202502137150Copyright © Аскаров М.С., Пак И.Л., Батенова У.Г., Ескермесов Д.Б., Клюев Д.А., Камышанский Е.К., 20252025Аскаров М.С., Пак И.Л., Батенова У.Г., Ескермесов Д.Б., Клюев Д.А., Камышанский Е.К.Askarov M.S., Pak I.L., Batenova U.G., Yeskermessov D.B., Klyuyev D.A., Kamyshanskiy Y.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://medecol.qmu.kz/jour/article/view/1005

Цель. Сравнительная морфометрическая, гистологическая и гистохимическая оценка эффективности комбинированного применения стромально-сосудистой фракции (SVF) и плазмы, обогащённой тромбоцитами (PRP) в лечении длительно незаживающих ран в эксперименте.Материалы и методы. Выполнено рандомизированное экспериментальное исследование на 32 кроликах. На спине животных были созданы эксцизионные раны диаметром 2 см. Животные случайным образом разделены на четыре группы: контрольная группа (антисептические препараты), группа с предоперационной обработкой физиологическим раствором (NaCl 0,9%), группа с предоперационной обработкой зоны планируемого оперативного вмешательства PRP и группа с предоперационной обработкой комбинацией PRP+SVF. Оценка эффективности проводилась морфометрическим, гистологическим и гистохимическим методами.Результаты и обсуждение. комбинированная терапия PRP и SVF способствовала ускоренному заживлению ран: усиление ангиогенеза, ускорению активного формирования внеклеточного матрикса.Выводы. Комбинированное применение PRP и SVF в значительной степени улучшает качество регенерации ткани и может быть эффективным методом терапии длительно незаживающих ран. 

Aim

Aim. Comparative morphometric, histological, and histochemical evaluation of the efficacy of combined application of stromal vascular fraction (SVF) and platelet-rich plasma (PRP) in the treatment of long non-healing wounds in an experi- mental model.

Materials and methods

Materials and methods. A randomized experimental study was conducted on 32 rabbits. Excisional wounds with a diameter of 2 cm were created on the animals' backs. The animals were randomly divided into four groups: control group (standard treatment), group with preoperative treatment using physiological saline, group with preoperative PRP+SVF injection, and group with both preoperative and postoperative PRP+SVF injections. The effectiveness was assessed using morphometric, histological, and histochemical methods.

Results

Results. The combined use of PRP and SVF enhanced angiogenesis, stimulated collagen synthesis, and reduced inflammatory cell infiltration.

Conclusion

Conclusion. The combined use of PRP and SVF significantly improves tissue regeneration quality and may be an effective method for the treatment of chronic wounds.

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The authors declare that there are no conflicts of interest present.